EVENT EMBRAPA 5.1 AND DETECTION METHODS

Plant: Bean (Phaseolus vulgaris)
Event: EMBRAPA 5.1
UI: Data not available
Commercial name: Data not available
Modification: rep (AC1) gene (antisense RNA: virus resistance)

bean plant pictureOriginal Cultivar and modification methods

The bean variety, named EMBRAPA 5.1, was produced by means of the biolistic technique and the pBGMVRNAiAHAS vector, transforming the cultivar Olathe Pinto.

Characteristics of the modification

Two partial sequences of the AC1 gene of the Bean Golden Mosaic Virus (BGMV), one with sense and the other with anti-sense orientation, were introduced into the genome of the EMBRAPA 5.1, allowing the GM plant to resist the virus by silencing the homonymous viral gene.
In the genome of the plant was also introduced a variant of the ahas gene (als) caming from A. thaliana, which normally provides tolerance to Imdazolinone herbicide. However, in the EMBRAPA 5.1 the protein AHAS was not detectable by ELISA analysis, so the plant is not tolerant to the herbicide Imidazolinone.

Purpose of the modification

To produce a bean variety resistant to BGMV virus.
Analysis methods based on nucleic acids

Protocol EMBRAPA 5.1, AZ et al. 2011

Documents
TitleYearAuthorJournal
RNAi-Mediated Resistance to Bean golden mosaic virus in Genetically Engineered Common Bean (Phaseolus vulgaris) 2007Kenny Bonfim et alMPMI Vol. 20, No. 6, 2007, pp. 717–726Read
Primer development for detection of Phaseolus vulgaris and Olathe transgenic bean2011Dinon AZ et al.R esumos do III Simpósio Brasileiro de Genética Molecular de Plantas 10 a 15 de abril de 2011 - Ilhéus - BA - BrasilRead


External links
 European Union Reference Laboratory for GM Food and Feed (EURL-GMFF)
 BCH - Living Modified Organism (LMO) Registry
 GMO Detection method Database (GMDD)
 OECD - BioTrack Product Database
 CropLife International Detection Methods

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Last update: 2018/02/12


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