EVENT MON87705 AND DETECTION METHODS

Plant: Soybean (Glycine max)
Event: MON87705
UI: MON-87705-6
Commercial name: Vistive Gold
Modification: fad2-1a and fatb1-a genes (antisense RNA: increased oleic acid), gene cp4epsps (herbicide tolerance: glyphosate)

soybean plant pictureOriginal Cultivar and modification methods

Soybean line MON87705 derives from the transformation of meristematic tissues of cultivar A3525 by means of Agrobacterium tumefaciens technique.
The plasmid vector PV-GMPQ/HT4404 contains two T-DNA: the first carries the cp4epsps transgene (aroA) and fragments of the FAD2 and FATB1 genes; the second carries fragments of the FAD2 and FATB1 genes in antisense orientation.


Characteristics of the modification

The genome of MON87705 soybean contains the DNA sequences present in the two T-DNAs.
As a consequence, the GM plant shows a minor expression of the FAD2 and FATB1 enzymes which results in an altered content of fatty acids with a considerable increase in oleic acid, a monosaturated fatty acid. In addition, the soybean MON87705 expresses la CP4 EPSPS protein which allows the plant to resist glyphosate.

Purpose of the modification

To produce a soybean variety with high oleic fatty acid and low saturated fatty acids, capable of tolerating glyphosate based herbicides.
Analysis methods based on nucleic acids

Protocol MON87705, Savini et al. 2012

Documents
TitleYearAuthorJournal
CONSENSUS DOCUMENT ON THE BIOLOGY OF GLYCINE MAX (L.) MERR. (SOYBEAN)2000OECDSeries on Harmonization of Regulatory Oversight in Biotechnology Read
Event-specific Method for the Quantification of Soybean MON 87705 Using Real-time PCR2012Savini et al. 2012Online Publication EURL-GMFFRead


External links
 European Union Reference Laboratory for GM Food and Feed (EURL-GMFF)
 BCH - Living Modified Organism (LMO) Registry
 GMO Detection method Database (GMDD)
 OECD - BioTrack Product Database
 CropLife International Detection Methods

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Last update: 2017/08/21


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