Maize MON810 GM EVENT AND DETECTION METHODS

Information
Detection Methods
Construct
Documents and Links

Plant:	Maize (Zea mays)
Event:	MON810
UI:	MON-00810-6
Commercial name:	Yieldgard
Modification:	cry1Ab gene (insect resistance: lepidoptera)

Original Cultivar and modification methods

The maize line MON810 was produced by means of the biolistic technique, transforming the genome of the maize plant with the plasmid vector PV-ZMBK07.

Characteristics of the modification

The genome of the maize line MON810 contains the cry1Ab gene, deriving from Bacillus thuringiensis subsp. Kurstaki.
The maize event MON810 expresses the Cry1Ab protein which, being toxic to Lepidoptera, allows the plant to resist Lepidoptera insect pests attacks.

Purpose of the modification

To produce a GM maize variety resistant, in particular, to Pyralid and Sesamia.

Analysis methods based on nucleic acids

	Protocol MON810, CRL-GMFF 2006
	Protocol TC1507-MON810-NK603-MON863-BT176-T25-GA21-BT11, Holck et al 2009

Analysis methods based on proteins

QualiPlate(TM) Kit for Cry1Ab/Cry1Ac

Certified reference materials

ERM, MON810 Maize

Construct MON810

Documents

Title	Year	Author	Journal
CRL assessment on the validation of an event specific method for the relative quantitation of maize line MON 810 DNA using real-time PCR as carried out by Federal Institute for Risk Assessment (BfR)	2006	-	-	Read
Detection of Recombinant DNA Segments Introduced to Genetically Modified Maize (Zea mays)	2002	Takeshi Matsuoka, Hideo Kuribara, Ken Takubo, Hiroshi Akiyama, Hirohito Miura, Yukihiro Goda, Yuko Kusakabe, Kenji Isshiki, Masatake Toyoda, and Akihiro Hino	J. Agric. Food Chem.	Read Read
CONSENSUS DOCUMENT ON THE BIOLOGY OF ZEA MAYS SUBSP. MAYS (MAIZE)	2003	OECD	Series on Harmonisation of Regulatory Oversight in Biotechnology	Read
TRANSGENIC PLANT EVENT DETECTION	2010	Van Den Bulcke et al	USPTO	Read Read
Qualitative and quantitative detection of genetically modified maize and soy in processed foods sold commercially in Brazil by PCR-based methods	2005	Ralf Greiner , Ursula Konietzny and Anna L.C.H. Villavicencio	Food Control	Read
Quantitative, multiplex ligation-dependent probe amplification for the determination of eight genetically modified maize events	2009	Askild Lorentz Holck, Signe M. Drømtorp and Even Heir	European Food Research and Technology Volume 230, Number 2, 185-194, DOI: 10.1007/s00217-009-1155-4	Read Read
Detection of Six Genetically Modified Maize Lines Using Optical Thin-Film Biosensor Chips	2010	Sulan Bai et al.	J. Agric. Food Chem., 2010, 58 (15), pp 8490–8494	Read
COMMISSION DECISION of 22 April 1998 concerning the placing on the market of genetically modified maize (Zea mays L. line MON 810), pursuant to Council Directive 90/220/EEC	1998	European Commission	Official Journal of the European Communities L 131/32	Read