EVENT MON810 AND DETECTION METHODS

Plant: Maize (Zea mays)
Event: MON810
UI: MON-00810-6
Commercial name: Yieldgard
Modification: cry1Ab gene (insect resistance: lepidoptera)

maize plant pictureOriginal Cultivar and modification methods

The maize line MON810 was produced by means of the biolistic technique, transforming the genome of the maize plant with the plasmid vector PV-ZMBK07.

Characteristics of the modification

The genome of the maize line MON810 contains the cry1Ab gene, deriving from Bacillus thuringiensis subsp. Kurstaki.
The maize event MON810 expresses the Cry1Ab protein which, being toxic to Lepidoptera, allows the plant to resist Lepidoptera insect pests attacks.

Purpose of the modification

To produce a GM maize variety resistant, in particular, to Pyralid and Sesamia.
Analysis methods based on nucleic acids

Protocol MON810, CRL-GMFF 2006
Protocol TC1507-MON810-NK603-MON863-BT176-T25-GA21-BT11, Holck et al 2009

Analysis methods based on proteins
QualiPlate(TM) Kit for Cry1Ab/Cry1Ac

Certified reference materials
ERM, MON810 Maize
Documents
TitleYearAuthorJournal
CRL assessment on the validation of an event specific method for the relative quantitation of maize line MON 810 DNA using real-time PCR as carried out by Federal Institute for Risk Assessment (BfR)2006--Read
Detection of Recombinant DNA Segments Introduced to Genetically Modified Maize (Zea mays)2002Takeshi Matsuoka, Hideo Kuribara, Ken Takubo, Hiroshi Akiyama, Hirohito Miura, Yukihiro Goda, Yuko Kusakabe, Kenji Isshiki, Masatake Toyoda, and Akihiro HinoJ. Agric. Food Chem.Read
Read
CONSENSUS DOCUMENT ON THE BIOLOGY OF ZEA MAYS SUBSP. MAYS (MAIZE)2003OECDSeries on Harmonisation of Regulatory Oversight in BiotechnologyRead
TRANSGENIC PLANT EVENT DETECTION2010Van Den Bulcke et alUSPTORead
Read
Qualitative and quantitative detection of genetically modified maize and soy in processed foods sold commercially in Brazil by PCR-based methods 2005Ralf Greiner , Ursula Konietzny and Anna L.C.H. VillavicencioFood ControlRead
Quantitative, multiplex ligation-dependent probe amplification for the determination of eight genetically modified maize events 2009Askild Lorentz Holck, Signe M. Drømtorp and Even HeirEuropean Food Research and Technology Volume 230, Number 2, 185-194, DOI: 10.1007/s00217-009-1155-4Read
Read
Detection of Six Genetically Modified Maize Lines Using Optical Thin-Film Biosensor Chips2010Sulan Bai et al.J. Agric. Food Chem., 2010, 58 (15), pp 8490–8494Read
COMMISSION DECISION of 22 April 1998 concerning the placing on the market of genetically modified maize (Zea mays L. line MON 810), pursuant to Council Directive 90/220/EEC1998European CommissionOfficial Journal of the European Communities L 131/32Read


External links
 GMOMETHODS: EU Database of Reference Methods for GMO Analysis
 European Union Reference Laboratory for GM Food and Feed (EURL-GMFF)
 BCH - Living Modified Organism (LMO) Registry
 EU register of genetically modified food and feed
 GMO Detection method Database (GMDD)
 CERA - GM Crop Database
 OECD - BioTrack Product Database
 CropLife International Detection Methods
 Read event description in the CERA database.

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Last update: 2014/09/29


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